Save the recording and allow mouse to recover.ĭata Analysis - Conscious Procedure with ECGenie.
Filtering signal in labchart reader software#
Monitor the body temperature with a rectal probe if possible.Alternatively insert needle electrodes subcutaneously into the limbs using Lead Configuration I: Left Arm – Right Arm (LA-RA) and/or Lead Configuration II: Left Leg – Right Arm (LL-RA). Fasten the mouse to the heated platform set to 37.5-39 o Add electrode cream if using the foot surface electrodes on the platform surface.Transfer mouse to a warmed platform and maintain with a nose cone at 2-2.5% isoflurane.Anesthetize with 2.5-4% isoflurane in oxygen. When saving sections with good readings, highlight the selected area and then save.For additional readings create a new session using the same settings as before.
After the desired acquisition time, (5-10 minutes) stop the reading.Place mouse on pad, lowering the Red Acrylic Cubby to surround the mouse on 3 sides discouraging escape.Open the ECG set up file (for default settings).Double click the icon ECG acquisition on the acquisition computer.Turn on the combined amplifier and the pre-amplifier tower.The plate will need to be covered with the extra cover in the package. The covering is removed to reveal three gel coated pads surrounded by a sticking plate. The lead plates are to be snapped into place onto the top of the pre-amplifier tower.
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